Note from Web Master (Often the word callus is used in this doc when the actual material is woundwood. You can look up the two words at www.treedictionary.com)
Journal of Arboriculture 9(1 2): December 1983 pg317
WOUND DRESSINGS: RESULTS OF STUDIES OVER
by Alex L. Shigo and Walter C. Shortle¹
Abstract.Many materials were used in and on experimentally inflicted wounds in many studies over a 13-year period. No material prevented decay. The individual tree had a greater effect on the wound than the treatments. Some individual trees of a species closed and compartmentalized wounds rapidly and effectively, regardless of treatment, while other trees did not close and compartmentalize treated or control wounds. The width of healthy wood behind wounds in red maple was the major factor affecting the course of the wound. Results are given from wounds on 275 treated and dissected trees.
Tree wound dressings do not prevent decay (Collins 1934, Marshall 1950, Shigo and Wilson 1977, Mercer 1979, 1982, Bonneman 1979, Dooley 1980), and are of limited benefit for wound closure (Neely 1970, McQuilkin 1950, Young and Tilford 1937).
Materials and Methods
During the 1970s, several wound treatment studies were conducted near Bartlett and West Thornton, New Hampshire, and Alfred, Maine. The studies were similar in general design, but there were some specific differences. Trees were wounded — with drill holes, ax cuts, or chisel wounds — some material was applied immediately after wounding (controls received no materials), the trees were cut after different periods of time, the wounds were dissected, closure was measured as width of open wound after periods of months to 7 years, microorganisms were isolated, and columns of discolored and decayed wood were measured. Tree species were red maple, Acer rubrum L, red oak, Quercus rubra L, white oak, Q. alba L, beech, Fagus grandifolia Ehrh.,
¹The use of trade, firm or corporation names in this publication is for the information and convenience of the reader; such use does not constitute endorsement or approval by the U.S. Department of Agriculture, or the U.S. Forest Service.
318Shigo & Shortle: Wound Dressings
and paper birch,Betula papyrifera. Trees ranged in age from 40 to 100 years, and in diameter at 1.4 m above ground from 15 to 30 cm. A total of 375 trees were wounded and treated, and 275 were harvested. (We always treat more than we plan to cut.) Materials used for most treatments were bituminous and asphalt-based paints, orange shellac, and nine variations of rosin acids and styrene butadiene, alone and with a synthetic thickener. Some preliminary studies were done with Ethrel, sucrose, cinnamic acid, aspirin, and ascorbic acid. The rosin acids were kindly supplied by chemists at Uniroyal Chemical Company, Naugatuck, CT. The ingredients were:
Rosin acid formulations
I.8619: Naugatex 1405 — Rosin acid emulsified, 50% styrene butadiene copolymer
II.Naugatex 2752: A 50% styrene butadiene acid terpolymer polymerized on alkyl aryl sulfonate emulsifier
III.Naugatex 3595: 60% styrene butadiene copolymer polymerized on a synthetic oligomeric surfactant
IV.Naugatex 9064: 23% styrene butadiene copolymer polymerized on sodium oleate emulsifier
V.Naugatex 3711: A 50% styrene butadiene acid terpolymer polymerized on a synthetic-oligomeric surfactant. This sample had 8 parts of rosin acid added after polymerization.
VI.Pyratex 1904: A butadiene styrene vinyl pyridine terpolymer polymerized on a Rosin Acid emulsifier.
VII. A 50% styrene butadiene copolymer polymerized on rosin acid emulsifier (Naugatex 2000). This sample had 1 part of a nonionic surfactant (Triton X-1001) added after polymerization.
VIII. A 50% styrene butadiene copolymer polymerized on a rosin acid (Naugatex 2000) to which 4 parts of rosin acid emulsifier was added after polymerization.
IX. A 50% styrene butadiene copolymer polymerized on a rosin acid (Naugatex 2000).
X. Synthetic thickener (Wica 6038) sodium polyacrylate thickening agent.
Cabot's wound healing paint was the bituminous-based dressing, and Treekote, a tar like material, was the other dressing. Ethrel is a plant regulator containing Ethephon (2-chlorethyl) phosphonic acid — 21.6%, and 78.4% inert ingredients. Ethrel used in experiments was in liquid form, 10% active ingredient. The orange shellac was a high quality commercial grade, the sucrose and cinnamic acid were high quality laboratory grades, and the aspirin was a common medical grade.
Here are some reasons for selection of materials: Orange shellac has a long reputation as a beneficial dressing. Treekote made no decay prevention claims on the container, and it was representative of the many tar like dressings. Cabot's paint did make claims, such as "heals wounds; prevents decay—stimulates growth of new bark—stops rot." Ethrel is used to loosen fruit for harvest, and to accelerate ripening. We wanted to determine its affect on injured tissues. Sugar was used because we hoped to stimulate the growth of microorganisms that might be beneficial for biological control of destructive wood-inhabiting microorganisms. Cinnamic acid is a protective chemical produced by trees after injury and infection. Maybe we could supply some extra cinnamic acid and help the tree help itself. The Uniroyal products were tried because of the firm bonding most of the materials had with fresh wounds, and because preliminary trials with one emulsified rosin acid appeared encouraging after a short time. Aspirin was used because the active ingredients are found in some trees. Maybe it could do for trees what it does for people. Ascorbic acid was used as a novelty.
Some specifics of ethrel treatments. On August 15, 1972, 15 trees, 5 each of red maple, American beech, and paper birch on the Hubbard Brook Experimental Forest near West Thornton, New Hampshire each received 12 drill hole wounds; 4 each at .5, 1, and 1.5 m above the ground. The wounds were spaced 90° from each other. The holes were 5 cm deep and 1.5 cm in diameter. Two holes at 180° from each other received 1 ml of liquid Ethrel. One tree of each species was cut on 16 October, 1972. Each wound was dissected longitudinally to measure the extent of discolored wood. Six wood chips,
Shigo & Shortle: Wound Dressings 319
3X8 mm, were extracted from above each wound and six from below. Chips were placed into malt-yeast agar (10 g malt extract, 2 g yeast extract, 1000 ml distilled water) for 10 days and the microorganisms identified when possible. The trees were observed periodically for 3 years, and in 1975 the remaining trees (only the beech were still standing) were cut and dissected.
Preliminary experiments with many substances.Wounds on red maples were treated with cinnamic acid (saturated aqueous solution), aspirin, 5% sucrose solution, and ascorbic acid.
Aspirin: 16 red maple trees at Bartlett, New Hampshire, were treated November 16, 1978; the procedure was the same for the ascorbic acid. Each tablet contained 5 grains of active ingredient. Five trees were cut October 10, 1980. Cracks were measured on November 22 and December 6, 1978.
Aspirin and ascorbic acid: 10 red maple trees, and 5 American beech trees at Bartlett, New Hampshire. Two and three whorls per tree, three drill holes per whorl; one hole control, one hole received two tablets aspirin, and one hole two tablets of ascorbic acid. Drill holes as in other experiments. Trees treated January 29, 1979; two trees harvested July 11, 1979.
Sugar: 20 red maple trees at Bartlett, New Hampshire, were treated with four template drill hole wounds per tree. The template was oval, 14 cm high and 10 cm wide; drills holes were evenly spaced, five of them in the form of a cross. Each hole was 3 cm deep and 1 cm wide. A sugar solution of 5% sucrose was painted over the wound and into the drill holes. Two wounds were treated and two not treated as controls on each tree. Seven trees were harvested; two on July 17, 1978, four in July, 1979, and one on October 28, 1980.
Cinnamic acid: The number of trees and wounds were the same as for sugar-treated trees. A solution of cinnamic acid was painted on two wounds of each tree. Six trees were harvested; two in July 1978, and four in July, 1979.
Rosin acids: 260 trees were treated; 170 red maple, 50 red oak, and 40 American beech. Of these, 165 were harvested; 115 red maple, 30 red oak, and 20 American beech, wounded from 1972 to 1974. Wounds were drill holes 5 cm deep, 1.5 cm wide, slanted downward slightly, and shallow oval wounds, 14 cm vertical and 10 cm horizontal. A template was used to make the size of the wound uniform. After the bark was removed, four horizontal chisel wounds .5 cm deep were made into the wood at a 45° angle downward. Four wounds of this type were inflicted on each tree. These wounds are called template-chisel wounds in this paper. Trees were harvested from 6 months through 3 years. To determine the area of cambial dieback, bark was removed from the wounded trunk and a centimeter grid was used. On some trees, the area of discolored wood on the cut surface 5 cm above the original wound was measured, also with a centimeter grid.
Cabot dressing: 10 trees each of red maple, red oak, and white oak were wounded on the Massabesic Experimental Forest, Alfred, Maine. Maples and white oaks were wounded on May 23, 1978, and red oaks on June 14, 1978. Five trees of each species were harvested on July 3,1979, and the remainder of the trees on June 16, 17, 1980. All trees received four wounds, two treated and two not treated as controls. Wounds were template type; bark was removed from an oval 14 cm long and 10 cm wide, and five drill holes, each 1.4 cm wide and 1 cm deep, were spaced equidistant on the wound surface in the form of a cross. This type of wound is called template-drill type in this paper. Measurements made for each wound were: closure as width between callus at center of wound, cambial dieback as cm2after bark removal, and length of discolored wood above and below wound. For isolation of microorganisms, 12 chips of wood were taken and placed in two culture plates within a medium of yeast and malt extract as defined in a
320 Shigo & Shortle: Wound Dressings
previous section. The area of discolored wood on the surface of a disk cut 5 cm above the edge of the original wound was measured.
Mixed treatments: 20 each red maple and red oak trees, maples on Hubbard Brook Experimental Forest, West Thornton, New Hampshire, and oaks on the Massabesic Experimental Forest, Alfred, Maine, were wounded and treated, the maples on August 23, 1976, and the oaks on August 24,1976. Treatments were Treekote, shellac, Uniroyal type I, and control. Each tree received four template-chisel type wounds. The lowest wound was .5 cm aboveground, and the other wounds were placed so that no wound was directly above or below another one. On September 3, all Treekote-treated wounds were treated as directed on the label. On July 13, 1977, 12 red maples were harvested, and areas of cambial dieback were measured. On July 12, five red oaks were harvested and the wounds were dissected to determine the amount of discolored wood behind the wounds.
On September 2, 1982, seven red oaks and seven red maple trees were harvested. After dissecting the trees, we measured cambial dieback about the wounds and the area of discolored and decayed wood on the face of 2 disks cut 5 cm above the top of the original wound. Microorganisms were isolated from 12 chips in two plates of malt-yeast agar as described in a previous section. On the red maple wounds the width of sound wood from the original wound to the margin of any internal column of defect was measured.
Ethrel-treated drill wounds. One tree each of red maple, paper birch, and American beech was cut 3 months after treatment. Trichoderma sp. was isolated frequently from treated wounds, but not from control wounds (Table 1). Treated wounds on red maple yielded many decay causing fungi, but only one wood chip from a control wound yielded a decay-causing fungus (Table
1). Species of Ceratocystis were common in all wounds. A Cytospora sp. was isolated only from red maple.
The average length of columns of discolored wood in the 3-month-old drill wounds was 1.5 cm for the four control wounds, and 8.0 cm for the four treated wounds (Fig. 1).
Ethrel-treated drill wounds were so decayed in the four red maple and four paper birch trees that the trees died and broke at the wound sites within 3 years.
The three remaining American beech trees were cut and dissected after 3 years. Fruit bodies of Panus stipticus were on decayed wood associated with treated wounds on the three trees (Fig. 2). Large columns of decayed wood were associated with all treated wounds (Fig. 3). All columns of decayed wood were similar in
ªEach tree had 3 whorls of drill wounds; 4 wounds, 2 treated and 2 control, per whorl. Twelve wood chips for isolation of microorganisms from each wound, 6 chips above wound and 6 below, 2 plates; malt-yeast agar, 60 wood chips for treated and 60 chips for control, per tree species. Relative occurrence = number of successful isolations/total isolation attempts X 100.
Journal of Arboriculture 9(12): December 1983 321
Preliminary experiments with many substances. Results of wound experiments with sugar solutions, cinnamic acid, aspirin, and ascorbic acid in red maple did not differ significantly from the controls.
After 1 year, the average lengths of columns of discolored wood associated with drill holes treated with ascorbic acid were 44 cm, treated, and 50 cm, controls (two trees, two control wounds, and six treated wounds).
After 1 year, the average lengths of columns of discolored wood associated with aspirin were 54 cm, treated, and 55 cm, controls (two trees, two control wounds, and six treated wounds).
After 1 year, the average lengths of columns of discolored wood associated with drill holes treated with aspirin, and other holes on the same tree treated with ascorbic acid, were 48 cm for aspirin, 52 cm for ascorbic acid, and 37 cm for controls (two trees, four holes for each treatment and controls).
After 1 year, the average lengths of columns of discolored wood associated with drilled template wounds treated with 5% sucrose solution were 40 cm, treated, and 40 cm, control (two trees, two control wounds and four treated wounds). Average dieback areas were 194 cm2 for controls and 225 cm² for sugar treated (four trees, eight controls, and eight treated wounds).
After 1 year, dieback areas on drilled template wounds treated with cinnamic acid were 583 cm² for controls, and 409 cm² for treated (three trees, six control and six treated wounds).
Other observations made on the wounded trees were measurements of cracks above and below the wounds, whether the wounds were oozing sap, and for how long. These factors appeared to be tree-related, more than treatment-related. When large vertical cracks appeared, they were associated with all wounds on a tree, treated and control. The same was true with oozing of sap after wounding and treatments. The genetic characteristics of individual trees within the same species were evident.
Rosin acids, Uniroyal products — short-term experiments. Preliminary experiments on red maple with rosin acid I, and several others were encouraging for reduction in area of cambial dieback over controls, and for decreases in length of discolored wood (Table 2). Harvests of red maple trees 6 and 12 months after treatment of drill wounds showed 9 to 56% less cambial dieback than in controls. Rosin acid I was one of the best materials in early tests that used all nine variations of the rosin acids and combination of the acids with the thickeners.
After 2 years, the results were different for red maples (Table 3). Decayed wood was associated with 9 control holes and 42 treated drill holes (all treatments except II and IV), and no treatment was significantly better than the control for discoloration (Table 3).
On red oak and American beech (Table 3), many treatments reduced discoloration associated with drill wounds, but after 2 years all the wounds were closed and only one treated wound had decayed wood.
The seven best treatments were used on template-chisel wounds on red maples (Table 4). The response of individual trees to the wounds was striking. All control wounds on some trees formed thick bands of callus (Fig. 4), while control wounds on other trees had large areas of dieback and very small bands of callus (Fig. 5). The same patterns were seen on trees with treated wounds. Some trees formed thick bands of callus (Fig. 6), and some trees did not (Fig. 7). There were slight decreases in cambial dieback associated with the treated wounds (Table 4) (Figs. 8 and 9). The amount of dieback appeared to be more tree related than treatment-related: large patches on some trees, (Fig. 8), and small patches on others (Fig. 9).
322 Shigo & Shortle: Wound Dressings
Click here for page 322 [figures 1-9]
Journal of Arboriculture 9(1 2): December 1983 323
Experiments with several treatments — short-term.
After many measurements on dissected trees, rosin acid I was selected in 1976 as
the best material, and new experiments were started to test it against a
commercial wound dressing (Treekote), orange shellac, and controls. On red
maple trees after 1 year, rosin acid I significantly reduced dieback area (Table
5) (Fig.10). On red oak very little dieback was associated with
any wounds (Fig. 11). On red maple the strong tree relationship to
wounding was seen again. On some trees, dieback was extensive about all
wounds (Fig. 12), while on other trees all wounds had little dieback (Fig. 13).
Wood-boring insects often infested the dying wood above and below the treated and control wounds (Fig. 13). The insect wounds extended greatly the area of cambial dieback.
Cabot dressing. In 1978, additional experiments were started with another, material, a bitunimous-base dressing called "Cabot's Tree Healing Paint." Template drill wounds were used. The black paint often peeled after one year (Fig.14). Control wounds blended with the bark color after 1 year on oaks (Fig. 15). On some trees, especially the white oaks, thick bands of callus formed on all wounds (Fig. 16). On other trees,
324 Shigo & Shortle: Wound Dressings
(Table 6.) (Table 7.)
especially red oaks and red maples, callus formation was poor on
all wounds (Fig. 17). After 1 year, the treated wounds on red oak had
smaller columns of discolored wood than the controls, but after 2 years there
was no difference (Table 6). The opposite was
true for red maples and white oaks. The treatment also reduced cambial
dieback on the oaks (Table 6). The treatment
had no effect on the relative frequency and occurrence of decay-causing
organisms in red maple, and red oak, but in white oak the treated wounds had a
significantly higher relative frequency and occurrence of decay-causing
organisms than the control (Table 6). When all
wounds on all three tree species were considered, there was no significant
difference in the number of decayed wounds by treatment — dressing or no
dressing (Table 7). Alternaria sp. was
isolated much more frequently from the treated wounds (Table
Experiments with several treatments — long term. After 7 years, seven red maple trees, and seven red oak trees, were dissected and studied. Each had four template-chisel wounds, each wound had four template-chisel wounds, each wound treated with either shellac, Rosin acid I, or Treekote, or not treated. On two red maple trees and two red oak trees, all four wounds were without decayed wood. For trees with decayed wood, there was no significant difference among treatments and controls (Table 8, Figs. 18 to 24). There were also no significant differences in area of dieback and area of discolored wood (Tables 8, 9; Figs. 18 to 24). The area of dieback was measured as the area of the still-exposed wood. This measurement reflects the opposite of closure.
Journal of Arboriculture 9(12): December 1983 325
Click here for Figures 10-18 as well as page 325.
326 Shigo & Shortle: Wound Dressings
The strong effect of the individual tree on
the wounds was shown again. One oak tree had closure of all wounds, and no
decay (Fig. 18), while another oak had poor closure
of all wounds and large pockets of decay (Fig. 19). Yet another tree had
poor closure on all wounds, and small pockets of decay (Fig. 20).
On red maples, some treatments were associated with large amounts of decayed wood, while no decayed wood was associated with the same treatments on other trees (Figs. 21 to 24). There was a strong correlation between decayed wood and the width of sound, healthy wood behind the wound at the time it was inflicted (Table 8, Figs. 25 and 26). Trees with large central columns of discolored and decayed wood had narrow bands of healthy wood, and new wounds inflicted at such locations developed decay rapidly. The data suggest for red maples, that if the width of sound wood behind a wound was less than 4 cm, the infection associated with the new wound spread rapidly (Table 8).
The wound response mechanisms of individual trees and their wounding history were the major factors affecting the patterns of infection started by new wounds, whether treated or not. Some individual trees of a species closed wounds and walled off infections rapidly and effectively, regardless of treatment. Other individual trees did not close wounds rapidly, and did not wall off infections to small volumes, regardless of treatment. The exception was the treatment with Ethrel which, under the conditions of the experiment, overwhelmed most of the trees, and encouraged rapid decay that led to breakage. None of the materials tested was better than the con-
Click here for Table 8
Journal of Arboriculture 9(12): December 1983 327
Click here for Page 327
328 Shigo & Shortle: Wound Dressings
trols for prevention of decay. Short-term experiments with
wound treatments can be very deceptive because decay usually takes several years
to develop. Any wound treatment study that does not produce decay in at
least 25% of the control wounds is of absolutely no value! Even in our
experiments, the short-term results were very different from the long-term
results, and our long term — 7 years — is still a very short time in the life of
Dissection of wounds is absolutely essential. Studies that do not dissect wounds confuse more than clarify. We have had too many of these.
When insects infested, they bored into the dying wood above and below the wound where there was no wound dressing.
On small wounds, closure was rapid and columns of discolored wood were usually small. Some treatments did reduce discoloration. Whether this is beneficial to the tree is questionable, because some types of discolored wood result from the accumulation of protective materials (Shortle 1979, Green et al. 1981). The white oaks in the Cabot paint study shed some light on this. The columns of discolored wood in treated wounds were significantly smaller than in the controls, yet there was significantly higher relative frequency and occurrence of decay causing fungi in the white oaks with treated wounds than in the controls. A similar trend was shown by Houston (1971), where columns of discolored wood were shortest in glue-treated wounds, but the incidence of decay was higher in glue-treated wounds in yellow birch.
The strong role of tree genetics in the wound response cannot be discounted. Several studies show that the ability of a tree to compartmentalize infections is under moderate to strong genetic control in several species (Shigo et al. 1977, Garrett et al. 1979, Lowerts and Kellison 1981). Trees that compartmentalize weakly will have larger columns of defect associated with dying branches and roots, and with mechanical wounds. The same individual tree that has had a life of poor compartmentalization will respond just as poorly to new wounds regardless of treatment. A summary of these conditions is given in Figure 27.
It is time to start a new direction with many tree care practices. New tools, new problems, new political pressures, and new information make it necessary to adjust many practices that were developed when conditions were different. New emphasis must be focused on the tree. If trees are well understood, then the ways to help them stay healthy will also be understood (Shigo 1982b). Ignorance is our main problem. Understanding is the answer. We should not rely on medicines and wound dressings to correct problems we create. It is not so important to start new practices as it is to STOP many old practices that do more harm than good. If adjustments can be made in many tree care practices, then there will be no need for dressings. For example, branch pruning has been a major tree treatment for centuries. The recommendation for 400 years has been to cut branches as close as possible to the joining stem, and then paint (Von Mayer-Wegelin 1936). If such cuts are made, the tree will be injured so seriously that even if a magic dressing were known, it would not help. If pruning cuts are made properly (Shigo1982a), there is no need for a dressing. If cuts are made improperly, dressings will not help.
In the end, arborists of Group I will continue to make adjustments based on new information. Group II will consider the new information and begin to make some adjustments. And, Group Ill will not understand what is happening because they will be out painting wounds.
We thank Mr. Wilson White and others at Uniroyal Corporation, Naugatuck, Connecticut for providing the rosin acids, and funds to support portions of the studies. We thank Cabot Company for providing wound dressings.
Click here for Literature Cited
Back to Hard To Get Docs